Guck in der Hilfe nach manual firmware upgrade
Avr studio-->help-->avr tools->isp mk2-->troubleshooting
Firmware upgrading is usually handled automatically by AVR Studio, if
the firmware distributed with AVR Studio is newer than the firmware
loaded into the AVRISP mkII.
However, the AVRISP mkII can stop responding to firmware upgrading if
the firmware on the AVRISP mkII is corrupted. Corruption of the firmware
can happen if the communication between the AVRISP mkII and the PC is
broken during firmware upgrading or if the firmware in the AVRISP mkII
is re-programmed with the wrong file.
Follow the steps below to manually upgrade the AVRISP mkII. This
procedure should work in all cases:
Disconnect the AVRISP mkII from target
Unplug the USB cable
Open the AVRISP mkII by pressing on the four plastic clips that holds
the top and bottom parts of the enclosure together.
Short-circuit pin 1 and pin 3 on the pinholes next to the AVRISP mkII
silk-print on the PCB. See figure below.
Insert the USB cable. The AVRISP mkII should now start blink red and
orange.
Start the application "AVRISP mkII Upgrade..." located in the "Tools"
menu in AVR Studio.
Click the "Start Upgrading" button.
When upgrade is finished, remove the short circuit between pin 1 and pin
3 on the AVRISP mkII.
Toggle AVRISP mkII power by unplug and re-insert the USB cable.
Press the "Done" button.
The AVRISP mkII is now upgraded and ready for use!